Name: A. Otten

Address: Universiteitssingel 50
Room number: 5.156
Telephone number: ++31 43 3881915
Job description: PhD student

Current work: Development of zebrafish models to study mitochondrial function and pathology of mitochondrial disorders.

2010-present             PhD student at department of Clinical Genomics

                                GROW School for Oncology and 
                                Developmental Biology,                                 Maastricht University, Maastricht

2008-2010                 Master in Cardiovascular Biology and
                                Cardiovascular Research Institute
                                Maastricht (CARIM),  Maastricht University,                                    Maastricht 
                                Main topics: Molecular Cell Biology, Clinical  

2010                        The PhD/Master course Advanced  Microscopy
                               and Vital Imaging
Maastricht University

2009                         Animal Course
                                Maastricht University

2009                         Course in Safe Labaratory Practice
                                Maastricht University

2005-2008                Bachelor in Molecular Life  Sciences, Faculty of
                               Health Medicine and Life Sciences Maastricht
                               University, Maastricht

During the senior internship of my master education I came in contact with the zebrafish model. Within the clinical genomics department I got the opportunity to use the zebrafish as a model for mitochondrial disorders, since the model can cope with the heterogeneous nature of these diseases and with the multiple genetic variants found in patients with mitochondrial dysfunction. We are optimizing tools and assays to be able to assess mitochondrial physiology in zebrafish both in health and disease (knockdown of genes and transgenesis). This will make the model ready for the study of genetic variants.

Furthermore, I use the zebrafish to unravel the mode of inheritance of mutations residing in the mitochondrial DNA. The high evolutionary mechanisms makes the zebrafish suitable to study this and to translate the findings to human situations.